US Long-Term Ecological Research Network

Using a peristaltic pump, water is pumped at a modest pace (< 15psi) through in-line filtration cartridges, loaded with 47mm, Millipore glass fiber filters. Filtered water volume is recorded. Cartridges are stored and transported in a cooler with ice.

In a dark, cool environment, filters are removed from the cartridges, folded in half (filtered material on the inside), placed in covered film canisters, and stored in a commercial freezer.  The canisters are removed from the freezer, and 25 mL of methanol is added to each. The canisters are capped, shaken lightly, and placed in the refrigerator for 24 hours. The methanol/chlorophyll solution is analyzed using a Turner Designs TD-700 fluorometer.

LTER Keywords
Protocol Format
Protocol ID
Protocol Type
field &amp; laboratory