US Long-Term Ecological Research Network
1. Label all chlorophyll, zooplankton, phytoplankton and acid blank samples (using pencil) with printed labels from LTER label templates on the CFL drive:
  • Put date on all samples requiring a written-in date
  • Put depth on zooplankton samples (if different from label)
2. Put pH samples in a room temperature water bath (i.e. small cooler with lid) to equilibrate samples to room temperature
3. Acidify Samples
  • use H2SO4 from SLOH for SLOH large bottles only (remember to mark bottle label after acidify)
  • use 1 mL of Optima HCl in S bottles and acid blanks (remember to remove all air from re-pipetter before adding HCl to first sample)
4. Put Glutaraldehyde in phytoplankton samples (3 partially full disposable pipette volumes per bottle). When done, rinse pipette with tap water and dispose of in Sharps Container
5. Refrigerate all LTER samples (except N samples go in freezer).   Zooplankton and phytoplankton samples are stored in Rm. 101A on proper shelf
6. Process Chlorophyll and TPM filters in dim light:
6a) Chlorophyll:
1) GFF fluorometer filters:
  • label film canister (include date and filter volume)
  • using forceps, fold filter in half (with filtered algae on inside) and place inside canister
  • make sure that top of film canister creates a good seal
  • put sample in freezer (keep all samples from same sampling week in separate bag)
2) GFF spectrophotometer filters:
  • label plastic tubes diagonally or vertically (include date and filter volume)
  • using forceps, fold filter in quarters and push down to bottom of tube with tweezers
  • add 5 mL of 90% acetone by volume (10% Milli-Q water). Before adding acetone, drain re-pipettor of air bubbles into waste. Check that the re-pipettor is set on 5 mL and periodically pump re-pipettor into graduated cylinder to confirm that it actually releases 5 mL of acetone.  
  • seal tube tightly with its cap
  • put sample in freezer in upright position in rack
6b) TPM
  • carefully fold filter in half using tweezers making sure the particulate matter is enclosed in the fold then place in the correctly numbered TPM filter holder (i.e. holder which filter originally came out of)
  • label container (include date and mL filtered)
  • place container in box in freezer (for later weighing)
7. pH
  • Check with LTER lab supervisor to ensure the pH meter is properly calibrated
  • Upon returning from sampling, place the pH samples in a room temperature light free water bath (use small cooler with lid)
  • remove parafilm from pH probe
  • aerate air-equilibrated samples vigorously for at least 5 minutes
  • wipe probe dry after its initial removal from solution and after every sample
  • after samples are brought to room temperature and air-equilibrated, place probe into sample and wait for the probe to stabilize then record pH and millivolts on data sheet
  • before discarding the samples, check to make sure that the data makes sense (compare to past data to see trends) and run samples again if necessary
  • when finished, replace parafilm to cover the hole and reinsert the electrode into the the electrode solution
8. SLOH Bottles: Before the 4:30 PM closing time, take the H bottles (acidified if necessary) in an ice-filled cooler along with the lab sheets (make sure the correct date and time is on lab sheets) to the SLOH on the east side (Agriculture Drive).
9. Cleaning Filter Cartridges (try to do the same day as sampled):
  • soak filter cartridges in Milli-Q or distilled water
  • if necessary, scrub filter paper off of filter holders
  • rinse cartridges with Milli-Q and refill with the appropriate new, clean filters 
Protocol ID