General Bottle Information:
- All bottles must be rinsed 3 times with sample water before being filled with sample water
- All scintillation bottles as well as the carbon vials should not contain air bubbles once filled with sample water. After filling with sample water, invert the bottle or vial and tap the bottom to check for rising air bubbles
- All Chemlab bottles must be coded with Chemlab labels. The N, A, and V bottles should be marked accordingly on the bottle caps.
- All Chemlab labels are to be copied (or a printed duplicate on regular print paper) and filed with field sheets in the appropriate binder to act as a record of bottle serial numbers.
H bottles:
- These are State Lab of Hygiene Bottles (SLOH)
- The H1 bottle is a 60 mL plastic bottle used for measuring ortho-phosphate and silica (no preservative added)
- The H2 bottle is a 250 mL plastic bottle used for measuring total phosphorus, total nitrogen, ammonia, and nitrate. This sample must be acidified using an H2SO4 (remember to mark bottle as acidified with H2SO4)
- These samples must be kept cold immediately after sampling in a cooler with ice
- These bottles must be delivered to the SLOH on the same day as sample collected (or the morning after sampling) with the accompanying lab sheets, one lab sheet per sampling depth. Be sure to enter the date and time the sample taken.
- The H bottles are labeled (using Sharpie) with lake code followed by depth:
Lake Code:
1 - Lake Mendota
2 - Lake Monona
3 - Lake Waubesa
4 - Lake Kegonsa
5 - Fish Lake
6 - Lake Wingra
Examples: 104 signifies a Lake Mendota 4 meter sample. 516 signifies a Fish Lake 16 meter sample
PH bottles:
- These are for measuring pH using the meter at the CFL
- There are 2 bottles used per sample depth, P1 is an air-excluded, 20mL scintillation vial and P2 is an air-equilibrated 125 mL bottle
S bottles:
- These are LTER nutrient bottles (total phosphorus, total nitrogen, nitrate, and silica)
- They are also used to measure cations during a quarterly sampling
- These bottles are filled to approximately 100 mL with sample
- They are always used in pairs, with one bottle filtered and one bottle unfiltered.
- All S bottles must be acidified with 1 mL of Optima HCl and remember to add acid to 3 acid blanks (labels for acid blanks are included in Fish Lake pH and chlorophyll labels). The acid blanks should be acidified at the same time as the samples and distributed amongst the samples during acidification.
N bottles:
- These are LTER 20mL scintillation vials used for measuring ammonia
- They will always accompany the S bottles (unless the S bottles are to be used for measuring cations only)
- The N bottle sample must be filtered
- They must be frozen as soon as the samples are brought back to the lab so never fill them totally full.
DIC/DOC tubes:
- These are 24mL, glass tubes for LTER carbon samples
- They are always used in pairs, with one tube filtered and one tube unfiltered
- Fill bottle entirely full so it is airtight
- Before sampling, while labeling and arranging bottles and tubes by lake and depth, write the tube numbers in the appropriate space on the field sheets.
V bottles
-These are LTER 20mL, scintillation vials used for measuring anions only during quarterly samplings
- The V bottle sample must be filtered
- Fill bottle entirely full so it is airtight
A bottles
- These are LTER 20mL, scintillation vials used for measuring alkalinity only during quarterly samplings
- The A bottle sample is unfiltered
- Fill bottle entirely full so it is airtight
Zooplankton bottles
- These are 110mL glass jars
Phytoplankton bottles
- These are 250mL SLOH nutrient bottles
Zebra Mussel bottles
- These are also 250mL SLOH nutrient bottles (see Zebra Mussel protocol, only sampled June-August)
LTER Keywords
Protocol ID
south_bottle_codes1
