1. Remove the samples from the shipper and refrigerate. If any samples are suspect upon receipt, make a note to be included with the analysis results.
2. Retrieve sample information from e-mail file or field sheet and place on top of the refrigerator.
Day of Analysis
1. Remove samples from refrigerator and warm to room temperature.
2. Prepare Standards. Record concentrations in Log Book at the top of the page.
3. Record the date, the analyst's initials, and client in the right hand corner under the Calibration Standard (CAL STD) information.
4. Check the sample vial numbers against vial numbers listed on the sheet provided by the client. Make note of any missing or mislabeled samples.
5. Record the sample numbers in the Log Book in the order of analysis.
a. Sample 1 will be reagent blanks. Record the average IC and OC values in mV of the last 3-5.
b. Samples 2-4 will be CAL STDs. Record the average OC value in mV.
c. Samples 5-6 will be Milli-Qs (MQ).
d. After every tenth sample, run a CAL STD and a MQ. Finish the run with a CAL STD and a MQ.
6. Organize CAL STDs, MQs, and samples and fill autosampler shuttles. Check shuttle order against Log Book order.
7. When the analytical run is finished, record the vial number in the right hand margin of the printout next to the corresponding IC/OC values. Indicate the reagent, MQ and CAL STD values which have been averaged. Cross out any outlying MQ or CAL STD values which have not been included in averaging.
8. In the Log Book, title two columns IC and OC. Record the results for each sample in ppm.
9. Calculate percent accuracy for each CAL STD.
a. Average the OC values of the Milli-Q readings in ppm except numbers 5-6.
b. Subtract the MQ average from each CAL STD. Divide by the Theoretical value for the CAL STD from Step 2.
c. Multiply by 100 to get a percentage.
Actual CAL STD value (OC in ppm) - MQ average (OC in ppm) X 100
Theoretical CAL STD value (in ppm)
d. Record the percent accuracy in the Log Book next to the corresponding CAL STD. The values should be within five percent of the theoretical value.
10. Round the IC/OC values to two decimal places. Transcribe the values onto the informational sheet or e-mail file.
11. After recording IC/OC values, check them against the values from the original printout.
12. Include your name and the date the results were sent at the bottom of the sheet or e-mail document.
13. Report Results.
a. If the results are to be reported by e-mail: save the data file; attach the data file to the letter; and print and file a hardcopy.
b. If the results are not to be reported by e-mail: make a photocopy of the data sheet; file the original data sheet; and file the photocopy in the folder labeled Carbon Data to be Sent.
Written by B. Fullington 11/15/94
Revised by J.A. Thoyre, and M.A. Schmidt 7/11/95