US Long-Term Ecological Research Network

Part of this research is about long-term heterotrophic bacterial community patterns in Lake Mendota, Wisconsin. Heterotrophic bacteria are responsible for nutrient cycling in aquatic systems. These preliminary abundance data indicate seasonal patterns in bacterial taxa (OTU’s). We will perform time-series analysis with these data together with environmental variables (water chemistry and meteorological factors) in order to investigate drivers of the bacterial community.

Relative abundance of bacterial community members from Lake Mendota, Wisconsin from 2000-2009 collected from an integrated 0-12m sample as part of the NTL-LTER Microbial Observatory. Samples were collected at approximately fortnightly frequency during ice-free seasons. Operational taxonomic units (OTU) ordered by base pair length of intergenic spacer region between the 16S and 23S bacterial ribosomal genes. Data were generated using automated ribosomal intergenic spacer analysis (ARISA) method (Fisher and Triplett, 1999). OTUs representing < 1% relative abundance indicated by white.

 

 


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NTL Microbial Observatory

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