(revised 6or28or02)NOTE: This protocol is for each sample site. Eight sites are sampled on each lake.Day CrewEquipment checklist:25 (50 halves) Minnow Traps and Floats Measuring board25 (50 halves) Crayfish Traps and Floats GPS25 slices Beef Liver (6.5 packages) Balances (5, 10, 30g)25 slices of white bread (2 bags) Computer16 Reflectors 12 volt batteriesForceps Data sheetsScale Envelopes and paper (write in rain) Full fuel tankID keys PFDsForceps OarsSmall Tubs AeratorsMinnow nets Measuring tapePlace 1 slice of white bread in each minnow trap and 1 slice of beef liver in each crayfish traps. Minnow traps have 2.5cm diameter openings and crayfish traps have 7.6cm openings.Locate the beginning of each site using the GPS.Set three minnow traps and three crayfish traps in shallow water (approx. 1 m), spaced approximately 15m apart along the 50m riparian transect corresponding with plots A, C, and E. Set the crayfish and minnow traps within two meters of each other.Traps are fished for approximately 24 hours. Crayfish are identified to species, counted per trap, and returned to the lake. Fish caught in either the crayfish or minnow traps are identified to species. Bluegill, pumpkinseed, rock bass, largemouth bass, smallmouth bass, yellow perch, and walleye are measured for total length, weighed and scales taken if necessary (see processing fish below). Any other species caught are identified to species, counted for each trap, and returned to the lake.After pulling the traps at each site, set out the reflectors for electrofishing. The reflectors should be placed 25m before the start of the adjacent riparian transect and 25m after the end of the transect.Night CrewEquipment Checklist:Fishboards (large and small) ElectrofishBoxBalances (5, 10, 30, 60, 100, 500, 1000 grams) ForcepsComputer HeadlampsScale envelopes Cliplights (2)Batteries (2-12 volts) Running lightsFull fuel tanks (generator and boat) AeratorsPFDs GPSOars Dip nets (2)Big Tubs (3) Small tubsRubber boots and gloves (2 pairs) Spotlight (2)Locate each site by finding the reflectors with the spotlight.Electrofish eight 100m transects on each lake after sunset.Follow a 1.5m depth contour along the shoreline, but make sure to electrofish near littoral structure (docks, cwd, etc).Two dipnetters will net all fish regardless of size and place them in the livewell.The driver should record the average DC electrical output in amps and the time taken to complete each transect on the driver datasheet.times Note – Communication between the driver and the netters is essential. It is the netters responsibility to let the driver know about obstructions (logs, rocks, etc) in the water and to let them know if they have to back up for missed fish. Dont be shy, the driver has to hear you over the generator.PROCESSING FISHSort fish into small tubs by species if necessary.Measure the total length (from nose to end of caudal fins pinched together) in mm and weight in grams for these seven species:Bluegill PumpkinseedLargemouth Bass Smallmouth BassRock Bass Yellow PerchWalleyeTake several scales from 5 fish of each of these species (bluegill, largemouth bass, smallmouth bass, yellow perch, and walleye) from each 10mm size class. Keep track of the number of samples taken from each of these species using the scale tally sheet.For yoy fish (for yoy lengths see Table 1), take lengths and weights of 30 fish of each of the above seven species per lake. If possible take several of the 30 required fish from different locations, not all from the same site.Weights should be taken with the appropriate sized spring balance – the fish should be in the mid-range of the scale.Record the date, lake code, site number, fish ID number, species, length, and weight on the scale envelope.Take the scales from behind the left pectoral fin if looking at the fish from the dorsal side. Place at least 5 scales in the scale envelope.Take the third dorsal spine from 5 yellow perch and bluegill for each age class (Table 1). Place it in the scale envelope.Identify all other fish and keep a count for each species for each trap or electrofish run.If a fish cannot be positively identified, preserve it for later identification.Revive fish that have not recovered by holding them by their dorsal surface in the water and gently rocking them to the left and right to move water across the gills.times Note - Remember to hang pesola spring balances to dry after each sampling. If the springs rust they are not reliable. Spring balances are to be calibrated weekly.