US Long-Term Ecological Research Network

North Temperate Lakes LTER Regional Survey Water Color Scans 2015 - current

Abstract
The Northern Highlands Lake District (NHLD) is one of the few regions in the world with periodic comprehensive water chemistry data from hundreds of lakes spanning almost a century. Birge and Juday directed the first comprehensive assessment of water chemistry in the NHLD, sampling more than 600 lakes in the 1920s and 30s. These surveys have been repeated by various agencies and we now have data from the 1920s (UW), 1960s (WDNR), 1970s (EPA), 1980s (EPA), 1990s (EPA), and 2000s (NTL). The 28 lakes sampled as part of the Regional Lake Survey have been sampled by at least four of these regional surveys including the 1920s Birge and Juday sampling efforts. These 28 lakes were selected to represent a gradient of landscape position and shoreline development, both of which are important factors influencing social and ecological dynamics of lakes in the NHLD. This long-term regional dataset will lead to a greater understanding of whether and how large-scale drivers such as climate change and variability, lakeshore residential development, introductions of invasive species, or forest management have altered regional water chemistry. Color is measured in water samples that are filtered in the field through 0.45 um nucleopore membrane filters. A spectrophotometer is used to quantify color in the lab as absorbance (unitless) at 1 nm intervals between the wavelengths of 200 and 800 nm. Absorbance data are considered suspect for values greater than 2.
Dataset ID
377
Date Range
-
LTER Keywords
Methods
We collect water samples for color at the deepest part of the lakes. The samples are surface water, filtered in the field through 0.45u polycarbonate membrane filters. We run a wavelength scan from 800 to 200nm, using a 5cm rectangular quartz cell in a Beckman Coulter Model DU800 spectrophotometer. Any samples that display absorbance values above 2AU are run again from 400 to 200nm using a 1cm quartz cuvette. Inititally the full range of wavelengths were run again and two values may be found in the database even if the original measurement with the large cuvette did not exceed 2AU. The user should discard values above 2AU and use values from the smaller cuvette instead. All values are given as measurements at the path lenth of the employed cuvette and need to be devided by the cuvette length for a comparable value at a pathlength of 1 cm.

The single beam Beckman Coulter DU800 spec is blanked first on a sample of DI water. Additional blank values are from a scan run on DI after that blanking as a check and are reported alongside the scans but are not subtracted from the scan values.
Version Number
3

North Temperate Lakes LTER: Color - Trout Lake Area 1989 - current

Abstract
Color is measured four times a year in the seven northern study lakes (Allequash, Big Muskellunge, Crystal, Sparkling, and Trout lakes, unnamed lakes 27-02 [Crystal Bog] and 12-15 [Trout Bog]) in water samples that are filtered in the field through 0.45 um nucleopore membrane filters. A spectrophotometer is used to quantify color in the lab as absorbance (unitless) at 1 nm intervals between the wavelengths of 200 and 800 nm (Note: Prior to August 2008, samples were initially run in 10cm cuvettes. Starting in Aug 2008, samples are initially run in 5cm cuvettes. Absorbance data are considered suspect for values greater than 2. Lakes with suspect data (typically, the bog lakes) are re-analyzed in the 200-400 nm range in 1 cm cuvettes. The data values are linear with respect to cuvette size. I.e, data from the 1cm cuvettes should be multiplied by 5 to be comparable with 5cm cuvette data and multiplied by 10 to be comparable with 10cm cuvette data. Data from 5cm cuvettes should be multiplied by 2 to be comparable with the older 10cm cuvette data. Sampling Frequency: 4 times annually Number of sites: 7
Dataset ID
87
Date Range
-
Maintenance
ongoing
Metadata Provider
Methods
We collect water samples for color at the deepest part of the lake four times per year: February under ice, spring mixis, August stratification, and fall mixis. The samples are surface water, filtered in the field through 0.45u polycarbonate membrane filters. We run a wavelength scan from 800 to 200nm, using a 5cm rectangular quartz cell in a Beckman Coulter Model DU800 spectrophotometer. Any samples that display absorbance values above 2AU are run again from 400 to 200nm using a 1cm quartz cuvette. Inititally the full range of wavelengths were run again and two values may be found in the database even if the original measurement with the large cuvette did not exceed 2AU. The user should discard values above 2AU and use values from the smaller cuvette instead. All values are given as measurements at the path lenth of the employed cuvette and need to be devided by the cuvette length for a comparable value at a pathlength of 1 cm.The single beam Beckman Coulter DU800 spec is blanked first on a sample of DI water. Additional blank values are from a scan run on DI after that blanking as a check and are reported alongside the scans but are not subtracted from the scan values. Protocol Log: 1990 -- we began running color scans, using a 10cm cylindrical cell with a Kontron spectrophotometer. 2001 -- we added scans in a 1cm cell for samples with high absorbance. July 2008 -- changed to Beckman Coulter DU800 spectrophotometer. At same time changed from 10cm to 5cm cell.
Short Name
NTLPH07
Version Number
31
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