US Long-Term Ecological Research Network

South: Bottle Codes

General Bottle Information:
-    All bottles must be rinsed 3 times with sample water before being filled with sample water
-    All scintillation bottles as well as the carbon vials should not contain air bubbles once filled with sample water. After filling with sample water, invert the bottle or vial and tap the bottom to check for rising air bubbles
-    All Chemlab bottles must be coded with Chemlab labels. The N, A, and V bottles should be marked accordingly on the bottle ca

Parameter: Manganese

Samples for manganese analysis (as well as dissolved nitrogen and phosphorus, silicon, calcium, sodium, potassium, magnesium, and iron) are collected together with a peristaltic pump and tubing and in-line filtered (through a 40 micron polycarbonate filter) into 120 ml LDPE bottles and acidified to a 1% HCl matrix by adding 1 ml of ultra pure concentrated HCl to 100 mls of sample. For every sample acidification event, three acid blanks are created by adding the same acid used on the samples to 100 mls of ultra pure water supplied from the lab.

Parameter: Iron

Samples for iron analysis (as well as dissolved nitrogen and phosphorus, silicon, calcium, sodium, potassium, magnesium, and manganese) are collected together with a peristaltic pump and tubing and in-line filtered (through a 40 micron polycarbonate filter) into 120 ml LDPE bottles and acidified to a 1% HCl matrix by adding 1 ml of ultra pure concentrated HCl to 100 mls of sample. For every sample acidification event, three acid blanks are created by adding the same acid used on the samples to 100 mls of ultra pure water supplied from the lab.

Parameter: Potassium

Samples for potassium analysis (as well as dissolved nitrogen and phosphorus, silicon, calcium, magnesium, sodium, iron, and manganese) are collected together with a peristaltic pump and tubing and in-line filtered (through a 40 micron polycarbonate filter) into 120 ml LDPE bottles and acidified to a 1% HCl matrix by adding 1 ml of ultra pure concentrated HCl to 100 mls of sample. For every sample acidification event, three acid blanks are created by adding the same acid used on the samples to 100 mls of ultra pure water supplied from the lab.

Parameter: Sodium

Samples for sodium analysis (as well as dissolved nitrogen and phosphorus, silicon, calcium, magnesium, potassium, iron, and manganese) are collected together with a peristaltic pump and tubing and in-line filtered (through a 40 micron polycarbonate filter) into 120 ml LDPE bottles and acidified to a 1% HCl matrix by adding 1 ml of ultra pure concentrated HCl to 100 mls of sample. For every sample acidification event, three acid blanks are created by adding the same acid used on the samples to 100 mls of ultra pure water supplied from the lab.

Parameter: Magnesium

Samples for magnesium analysis (as well as dissolved nitrogen and phosphorus, silicon, calcium, sodium, potassium, iron, and manganese) are collected together with a peristaltic pump and tubing and in-line filtered (through a 40 micron polycarbonate filter) into 120 ml LDPE bottles and acidified to a 1% HCl matrix by adding 1 ml of ultra pure concentrated HCl to 100 mls of sample. For every sample acidification event, three acid blanks are created by adding the same acid used on the samples to 100 mls of ultra pure water supplied from the lab.

Parameter: Calcium

Samples for calcium analysis (as well as dissolved nitrogen and phosphorus, silicon, magnesium, sodium, potassium, iron, and manganese) are collected together with a peristaltic pump and tubing and in-line filtered (through a 40 micron polycarbonate filter) into 120 ml LDPE bottles and acidified to a 1% HCl matrix by adding 1 ml of ultra pure concentrated HCl to 100 mls of sample. For every sample acidification event, three acid blanks are created by adding the same acid used on the samples to 100 mls of ultra pure water supplied from the lab.
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