Samples for sulfate and chloride are collected together with a peristaltic pump and tubing and in-line filtered (through a 0.40 micron polycarbonate filter) into new, 20 ml HDPE plastic containers with conical caps. They are refrigerated at 4 degrees Celsius until analysis, which should occur within 6 months.

Samples for chloride and sulfate are collected together with a peristaltic pump and tubing and in-line filtered (through a 0.40 micron polycarbonate filter) into new, 20 ml HDPE plastic containers with conical caps. The samples are stored refrigerated at 4 degrees Celsius until analysis, which should occur within 6 months.

Samples for total and dissolved phosphorus and nitrogen analysis (as well as dissolved silicon, calcium, magnesium, sodium, potassium, iron, and manganese) are collected together with a peristaltic pump and tubing and in-line filtered, when necessary, (through a 40 micron polycarbonate filter) into 120 ml LDPE bottles and acidified to a 1% HCl matrix by adding 1 mL of ultra pure concentrated HCl to 100 mls of sample. For every sample acidification event, three acid blanks are created by adding the same acid used on the samples to 100 mls of ultra pure water supplied from the lab.

Samples for total and dissolved nitrogen and phosphorus analysis (as well as dissolved silicon, calcium, magnesium, sodium, potassium, iron, and manganese) are collected together with a peristaltic pump and tubing and in-line filtered, when necessary, (through a 40 micron polycarbonate filter) into 120 ml LDPE bottles and acidified to a 1% HCl matrix by adding 1 mL of ultra pure concentrated HCl to 100 mls of sample. For every sample acidification event, three acid blanks are created by adding the same acid used on the samples to 100 mls of ultra pure water supplied from the lab.

Samples for silicon analysis (as well as dissolved nitrogen and phosphorus, calcium, magnesium, sodium, potassium, iron, and manganese) are collected together with a peristaltic pump and tubing and in-line filtered (through a 40 micron polycarbonate filter) into 120 ml LDPE bottles and acidified to a 1% HCl matrix by adding 1 ml of ultra pure concentrated HCl to 100 mls of sample. For every sample acidification event, three acid blanks are created by adding the same acid used on the samples to 100 mls of ultra pure water supplied from the lab.

Samples for orthophosphate, nitrate/nitrite and ammonium are collected together with a peristaltic pump and tubing and in-line filtered (through a 40 micron polycarbonate filter) into new, 20 ml HDPE plastic containers with conical caps. The samples are stored frozen until analysis, which should occur within 6 months of freezing. The samples are analyzed for orthophosphate (nitrate/nitrite, and ammonium simultaneously) by automated colorimetric spectrophotometry, using a segmented flow autoanalyzer.

Samples for nitrate/nitrite and ammonium are collected together with a peristaltic pump and tubing and in-line filtered (through a 40 micron polycarbonate filter) into new, 20 ml HDPE plastic containers with conical caps. The samples are stored frozen until analysis, which should occur within 6 months. The samples are analyzed for nitrate/nitrite (and ammonium) simultaneously by automated colorimetric spectrophotometry, using a segmented flow autoanalyzer.

Samples for ammonium and nitrate/nitrite are collected together with a peristaltic pump and tubing and in-line filtered (through a 0.40 micron polycarbonate filter) into new, 20 ml HDPE plastic containers with conical caps. The samples are stored frozen until analysis, which should occur within 6 months. The samples are analyzed for ammonium (and nitrate/nitrite) simultaneously by automated colorimetric spectrophotometry, using a segmented flow autoanalyzer.

Samples for inorganic and organic carbon are collected together with a peristaltic pump and tubing and in-line filtered, if necessary, (through a 0.40 micron polycarbonate filter) into glass, 24 ml vials (that are compatible with the carbon analyzer autosampler), and capped with septa, leaving no head space.

Samples for alkalinity are collected with a peristaltic pump and tubing into new, 20 ml HDPE plastic containers with conical caps. The samples are stored refrigerated at 4 degrees Celsius until analysis, which should occur within 2 weeks. The samples are warmed to room temperature and then analyzed with an Orion 720A pH meter and Radiometer combination electrode. The sample is titrated to an endpoint pH of approximately 3.557 by adding 0.05N HCl to 16 mls sample at the Hasler Lab (or 0.01N HCl to 4 mls sample at Trout Lake Station Lab) in 10 microliter increments using a micro-pipette.